|
Cloned
pets a step nearer as cat is cloned
THE LONG-CHERISHED dream of cloning much-loved family pets came
a step closer last week, when it was announced that American
scientists have succeeded in cloning a domestic cat for the
first time. The two-month-old tortoiseshell kitten, called Cc
- short for carbon copy - appears to be healthy,
writes Nick Mays.
Cats are the sixth mammal - after sheep, cows, mice, goats and
pigs - to be cloned with the nuclear transfer technique used
to produce Dolly the sheep.
However, as with all the previously cloned species, the cloning
success rate is low. Cc was the only live birth out of 87 cloned
embryos implanted into surrogate mothers.
Although Cc is a genetically identical twin of the donor cat,
it is far from a carbon copy. The pattern on cats coats
is only partly determined by genes; it also depends on the environment
in the womb as the kitten embryo grows. Ccs colouring
is unique.
Cats personalities, as with those of any cloned animal,
are also likely to be heavily influenced by conditions inside
the womb and during their first few months, making an exact
replica of the original animal still something confined to the
realms of science fiction.
The female kitten was created by Dr Mark Westhusin and colleagues
at Texas A&M University in College Station, home of the
much-vaunted Missyplicity Project which has been
trying, for the past five years, to create a cloned dog.

The
first-ever cloned cat, shown here at seven weeks old,
with Allie, her surrogate mother.
The
team took DNA from an adult female tortoiseshell cat named
Rainbow and injected it into a hollowed-out egg
cell whose nucleus had been removed.
The egg was then kick-started into life with an
electric shock and implanted into a surrogate tabby mother.
The experiment, reported in the journal Nature, was paid for
by John Sperling, an 81-year-old financier who owns a company
called Genetic Savings & Clone. Mr Sperling says he aims
to offer the technology to wealthy people seeking to clone
their favourite pet animals, and wants to charge pet owners
to clone their animals.
Cloned cats are expected to attract a price tag of around
£7,000, although the price is expected to fall to around
£700. Cloned dogs will be much more expensive, with
£70,000 being the initial price, possibly dropping to
a mere snip at £5,000.
Despite the hype, however, the success rate will have to be
improved if pet cloning is to be a reality and highlights
once again the dangers of cloning. Cloned animals also tend
to be larger than normal and have been shown to suffer from
immune system problems.
Tests have shown that cloned animals have shorter than normal
lifespans and are unusually vulnerable to disease, according
to research published in January. Scientists in Tokyo have
shown that cloned mice die significantly earlier than mice
conceived naturally. They also have weakened immune systems
and suffer from pneumonia, tumours and liver failure.
The new mouse research was led by Dr Atsuo Ogura at the National
Institute of Infectious Diseases in Tokyo. It examined 12
mice cloned from testes cells. The cloned mice began to die
after 311 days, with 10 dying before 800 days. Out of a control
group of seven mice that were conceived naturally, only one
died before 800 days.
When the scientists examined six of the dead clones all had
severe pneumonia, two had cancer and four had liver failure,
they report in Nature Genetics. Their immune systems were
also weak. The clones were created in the same way as Dolly.
A cell nucleus and its DNA were removed from a donor cell
and placed inside an unfertilised egg, from which its nucleus
had been removed.
It was disclosed a few weeks ago that Dolly the sheep, the
first mammal to be cloned from an adult, had arthritis in
one leg, a possible symptom of premature ageing. Dolly was
the only healthy live-born lamb out of 277 attempts at the
Roslin Institute, Edinburgh. Earlier this year, Prof Ian Wilmut,
one of the scientists who cloned Dolly, called for a full
inquiry into the health of all cloned animals.
Premature ageing
In
1999 researchers found evidence that she may be ageing prematurely.
Scientists at PPL Therapeutics, the company that worked with
the Roslin Institute to create Dolly, looked at structures
in her cells called telomeres. Made from protein and nucleic
acid, these cap the ends of chromosomes, protecting them from
fraying.
Telomeres have been linked to the natural life cycle of cells.
Each time a cell divides, the telomeres become shorter until,
after a predetermined number of divisions, they crumble away,
allowing the cell to die. Dollys telomeres appear to
be shorter than normal, suggesting that she may be genetically
older than chronological age.
Despite the ongoing concerns over the drawbacks of cloning,
the scientists at Texas A & M remain upbeat about Ccs
successful birth and the possibility of cloned pets at last
becoming a reality for pet lovers worldwide.
A kitten was delivered by caesarean section on Dec 22,
2001, 66 days after the embryo was transferred, said
a team spokesperson. The kitten was vigorous at birth
and appears to be completely normal.
Tests carried out after Ccs birth confirmed that the
kitten was a clone - genetically identical to the donor.
|