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Dog study may lead to new epilepsy treatment

EPILEPTICS MAY benefit from new treatments after scientists discovered why the condition is common in some dog breeds. Purebred Miniature-Wirehaired Dachshunds, which suffer a form of epilepsy similar to one called Lafora disease in humans, share a mutation in a gene, an international team has reported in the journal Science.

The finding has allowed the researchers to develop a test that could soon help owners breed out the disease. The latest development, reported in Science magazine, is an example of how the human and dog genome projects are expected to benefit both species. Researchers are comparing and contrasting the "life codes" of the two mammals with other animals to track down the genetic causes of ill health.

The study in Science was produced by a Canadian/UK team led from the Hospital for Sick Children (HSC) in Toronto. The researchers showed that the jerky behaviour and seizures suffered by purebred miniature wirehaired dachshunds were caused by a form of epilepsy called EPM2.

The affected dogs all share a mutation in their EPM2b gene involving multiple repeats in the DNA code that prevent the proper production of protein. It is thought 5% of Miniature Wirehaireds in the UK have the disease and perhaps as many as 25% may be carriers of the faulty gene.

Owners usually start to notice a problem with their pets when they are about six years old. Although incurable, the disease can be managed with a controlled diet and drugs. "These animals will jerk in response to quite specific things, such as sudden movement in their visual field," said Science co-author Clare Rusbridge, a veterinary neurologist at the Stone Lion Veterinary Centre in London.

"They also do it when there is flickering light - this is one of the photosensitive epilepsies. One of the simplest managements is doggy sunglasses, which means they can be walked and enjoy life."

Severe

If dogs can cope with EPM2, the same cannot be said of humans suffering with Lafora disease. It is the most severe form of teenage-onset epilepsy. It gets progressively worse and usually results in death within a few years of the diagnosis.

"In terms of frequency, it is very rare but it is a horrible disease," said colleague Dr Berge Minassian, whose research group at the HSC has now identified two faulty genes associated with Lafora's. "The seizures get more and more frequent and severe, and within a year or two they are totally uncontrollable by any means."

For dogs the benefits are more obvious. With a new test for the faulty EPM2b gene, breed clubs could soon start a programme of controlled mating to eradicate the disease in Miniature Wirehaireds and other breeds, such as basset hounds, in which it has become amplified. This approach is already being used in Irish setters, for example, to tackle a blinding condition known as progressive retinal atrophy (PRA), and an immune disorder called canine leukocyte adhesion deficiency (Clad). Just like EPM2, both are the result of recessive mutations - a dog must have two copies (one from each parent) of a "bad" gene to show the disease.

The Kennel Club is about to stop registering any Irish setter unless it is clear of Clad. "Because purebred dogs are highly selected - breeders choose the dams and sires they put together - we can impose restrictions to select against dogs that are likely to pass these genes on to future generations," commented Dr Jeff Sampson, the Kennel Club's Canine Genetics Co-ordinator.

There is great hope that purebred dogs, with their large litters and long pedigrees, will offer science the opportunity to rapidly locate faulty genes that in humans would be far more difficult to find because few family members may be alive to study their DNA.

"Human clinicians are increasingly turning to purebred canine populations because these will have similar, if not identical, diseases to us and the clinicians will identify the gene in the dog and that will then give them a handle to start looking in human populations," explained Dr Sampson.

The Toronto hospital research group is already searching for other instances in which the particular pattern of expanded DNA repeats seen in the dachshunds may be driving ill health.

"Our discovery can now help to eliminate the disease by being able to diagnose carrier and affected dogs even at birth," said Dr Berge Minassian of the Hospital for Sick Children, Toronto."

We are learning from some successful experiences in helping to reduce the severity of seizures in the dogs and applying them to humans."

He added that the dogs would prove a valuable testing ground for new drugs to treat affected people." We’ve gone on to check the human genome as well as the genomes of cattle and other species and have found a number of genes that contain such repeats, and we are in the process of figuring out if they are associated with diseases."


We have exciting veterinary news from Canada.  Researchers at the renowned Hospital for Sick Children in Toronto, Canada have identified a gene believed to be responsible for a rare form of epilepsy found in dogs.   

Dr. Berge Minassian,   a neurologist at the hospital, reports the finding of the first dog epilepsy gene which helps to explain the high incidence of epilepsy in dogs. The rate in dogs is five per cent compared with one per cent in humans. 

 The discovery came about during research into a child form of epilepsy known as Lafora disease. This appears during teen age years. The seizures are serious and can be fatal. Currently available medications are not effective. There was a need to find an animal model and Dr Minassian was aware that dogs suffered from autosomal recessive myoclonic epilepsy (PME).

 This is particularly common in Basset Hounds, Standard Poodles, Pointers, Corgis, Beagles and Dachshunds.  Both dogs and people who suffer this form of epilepsy can be subject to easily provoked seizures and are very light sensitive.

 Currently further research is taking place in an effort to develop a commercially available test to identify the gene and thus eliminate it through controlled breeding practices.

Clive Davies